Wireless Fiber Photometry

Obtain precise real-time measurements of neural circuits during operant conditioning.

Fiber photometry is a powerful ultra-fast technique used to measure calcium, neurotransmitters, and other molecules in vivo. To use fiber photometry, genetically encoded calcium indicators (GECIs), dopamine indicators (DLIGHT1), norepinephrine indicators (GRABNE), and other fluorescent sensors are first expressed at the location of interest. An optical fiber is then used to excite the sensor and detect the resulting fluorescence.

But when used in freely moving animals, along optical cable attached to the head of the animal can be problematic. The cable can impede movement in environments containing exercise wheels or shelters, and also alter social interactions. The cable can also cause artifacts when used with video tracking systems. For example, the cable often continues to sway after the animal has stopped moving, making it difficult for software to recognize freezing behavior. Placing all components on a headstage ends all of these problems.

 

TeleFipho includes all of the components required for fiber photometry – optical fiber, filter cube, light source, and photodetector – as well as wireless transmission hardware, all in a 3-gram headstage.

TeleFipho definitely will not block the free behavior of your animals, enabling novel experimental approaches using fiber photometry.

Features

  • World-first commercial wireless fiber photometry
  • Small headstage / good for mice, rats, marmosets, etc.
  • Standard 2.5 mm ferrule cannula
  • Rechargeable by a dedicated charger
  • Adjustable excitation LED power
  • Adjustable signal offset
  • For GCaMP or GFP-like indicators
  • Half the price of traditional fiber photometry systems!

Simple setup – headstage, receiver and software

Stress-induced GCaMP signal from orexin neurons in mouse hypothalamus. By courtesy of Dr. Daisuke Ono in Akihiro Yamanaka Lab, Nagoya University.

TeleFipho Standard Set

TeleFipho Standard Set consists of the following items:

  • TeleFiT 1x TeleFipho Transmitter Headstage
  • TeleFiR 1x TeleFipho Receiver
  • TeleFiCharger 1x TeleFipho Charger
  • TeleFiC_x 3x TeleFipho Cannula
  • TeleFiTool 1x Insertion Tool for TeleFipho
  • TeleFiDummy 1x TeleFipho Dummy Headstage
  • 1x TeleFipho software installer

(※) Please specify cannula length.

Stand Alone / External Recorder

TeleFipho receiver can be directly connected to PC via USB for fiber photometry data recording in TeleFipho software. TeleFipho software is good enough for simple raw data / low pass filtered data recording.

Data can be directly read from Matlab / Octave or can be exported to ASCII format.
For more complicated online analysis and/or parallel recording of other physiological data, you can access photometry data as an analog signal via BNC on the front panel of the TeleFipho receiver so that you can connect the external data recording system (e.g. PowerLab from ADI).

Specifications

Headstage Weight 3 g
Headstage size 12 x 12 x 22 mm
Excitation wavelength LED peak 470 nm, Filter band 445~490 nm
Emission wavelength Filter band 500~550 nm
Excitation power 10~300 µW @ Fiber end (Adjustable)
Sampling rate 100Hz
AD resolution 16 bit
Photo sensor Photo diode
Gain 1010 V/A
Battery life 2 hours @ Excitation power 30 µW
Transmission band 2.4 GHz
Transmission distance 2 m
Power Battery powered, rechargeable
Receiver I/O 1x Photometry analog out, 1x General purpose analog In (0~5V)
PC Interface USB / TeleFipho software (for Windows 10)
Cannula core: 400 µm / NA 0.39, Cladding: 425 µm, Ferrule: 2.5 mm
Model Description
TeleFipho-set TeleFipho Standard Set
TeleFiT TeleFipho Transmitter Headstage
TeleFiR TeleFipho Receiver
TeleFiCharger TeleFipho Charger
TeleFiC_x TeleFipho Cannula
TeleFiTool Insertion Tool for TeleFipho
TeleFiDummy TeleFipho Dummy Headstage

Let’s Get Started!

Virtual demos available.