Richell Microresico® Low Bind 96-well Plate

Peptide Binding Analyses using HPLC

Method

1) Incubate each tube with Trypsin-digested BSA for 24h.
2) Collect and concentrate the unbound peptides.
3) Dissolve each concentrate with 0.1% TFA/H2O.
4) Analyze by Nano RP-HPLC.
Control: Trypsin-digested BSA diluted with 0.1 TFA/H2O.

Protein Binding Analyses using ELISA

Method

1) Incubate each tube with Adiponectin (0.75ng/ml in PBS) for 3h.
2) Quantify the unbound Adiponectin by ELISA to determine loss rate.

Fluid Recovery through Pipetting

Method

1) Incubate each tube with dye-solution (Bordeaux S) for 1h.
2) Remove the dye-solution from tube by pipetting, add 0.5ml distilled water to the tube, and analyze the absorption of the dye to determine the residual rate.