Microresico® features a low level of peptide or protein absorption to the container wall without the need for coating.
With Microresico®, you can maximize recovery of fluid samples.
Peptide Binding Analyses using HPLC
1) Incubate each tube with Trypsin-digested BSA for 24h.
2) Collect and concentrate the unbound peptides.
3) Dissolve each concentrate with 0.1% TFA/H2O.
4) Analyze by Nano RP-HPLC.
Control: Trypsin-digested BSA diluted with 0.1 TFA/H2O.
Protein Binding Analyses using ELISA
1) Incubate each tube with Adiponectin (0.75ng/ml in PBS) for 3h.
2) Quantify the unbound Adiponectin by ELISA to determine loss rate.
Fluid Recovery through Pipetting
1) Incubate each tube with dye-solution (Bordeaux S) for 1h.
2) Remove the dye-solution from tube by pipetting, add 0.5ml distilled water to the tube, and analyze the absorption of the dye to determine the residual rate.
Material: Polypropylene, Non sterile
Dimension: 86 x 128 x 14.4 mm
Autoclavable: 121℃ 20 min