In Vitro Optogenetics

Wireless Optogenetic Stimulator

Optogenetics

In-vivo Optogenetics has become explosively popular for studying animal behavior. Now this technology is increasingly being used in vitro with multiwell plates and cell culture dishes, applied to studies of gene expression, signalling pathways, protein clustering, and other topics. 

For this purpose, long-term and time-controlled light stimulation in a culture incubator is required. This incubator-compatible LED array fulfills all the requirements for in-vitro optogenetics experiments

    LED Array System 

    • 96 well plate format – Customizable to different sizes and layouts
    • Use in your incubator: compatible with humid and high CO2 environments
    • Compact: 144 x 102 x 22 mm
    • Many Color Options
    • Multi-color arrays
    • Fully programmable pulse timing and irradiance
    • Deliver light from above or below plates.
    • Customizable for different size plates and different well layouts

    We use it routinely, use it every day. We  love working with it, it’s perfect for us.
    It’s our high throughput system.

    Dr. Jorine Eeftens

    Brangwynne Lab, Princeton University

    Wavelength Availability

    • White
    • UV  280 nm, 365 nm
    • Violet 405 nm, 420 nm
    • Blue 450 nm, 470 nm

  • Green 525 nm
  • Yellow 590 nm
  • Red 630 nm, 660 nm, 740 nm
  • Infra-Red 940 nm
  • Two color arrays are also available for alternating stimulation and inhibition.
     

    LAD Array Drivers

    The LAD1 and LAD4 drivers power our arrays. Pulse intensity and timing can be directly controlled by an analog input. The LAD can also be set to deliver constant illumination.

    The LAD4 and LEDA4 allows you to split a 96 well plate into 4 sectors and illuminate each sector independently.

    Pulse Generator for Optogenetics

    STOmk-2 is a pulse generator developed for optogenetics. By connecting STOmk-2 to TRG port on the Teleopto Remote Controller via a trigger cable, you can control the timing of light stimulation by TTL pulses. Pulses are defined by the parameters illustrated below.

    Currently being used to study:

    • Stem cell (IPSC) differentiation
    • Developmental biology 
    • Molecular biology – receptor directed protein expression (via Cry2 activation)

    • CRISPR/Cas9 gene modification
    • Oncology 
    • Opthalmology & opthalmologic drug development
    • Photostimulation and bleaching of proteins

    Please see our optogenetic resource guide to help plan your experiments.

    Publication

    Zhu, L., Richardson, T. M., Wacheul, L., Wei, M. T., Feric, M., Whitney, G., … & Brangwynne, C. P. (2019). Controlling the material properties and rRNA processing function of the nucleolus using light. Proceedings of the National Academy of Sciences, 201903870.

    Westergard, T., McAvoy, K., Russell, K., Wen, X., Pang, Y., Morris, B., … & Haeusler, A. (2019). Repeat‐associated non‐AUG translation in C9orf72‐ALS/FTD is driven by neuronal excitation and stress. EMBO molecular medicine11(2), e9423.

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