In Vitro Optogenetics

Optogenetics

In-vivo Optogenetics has become very popular for studying animal behavior. This technology is now used more and more in vitro with multiwell plates and cell culture dishes. Applied to studies of gene expression, signaling pathways, protein clustering, and other topics.

For this purpose, long-term and time-controlled light stimulation in a culture incubator are required. This incubator-compatible LED array fulfills all the requirements for in-vitro optogenetics experiments

LED Array System 

  • LED Microplate Illumination
  • 96 well plate format – Customizable for different multiwell plates and layouts
  • Light delivery in your incubator: compatible with humid and high CO2environments (sp)
  • Compact: 144 x 102 x 22 mm
  • Many Color Options
  • Multi-color arrays
  • Fully programmable pulse timing and irradiance
  • Compatible: Can be triggered by TTL signals from your equipment
  • Deliver light from above or below plates.

You can read more about LEDA arrays here: Illuminating Cells in Microplates

Please see our optogenetic resource guide to help plan your experiments.

We use it routinely, use it every day. We  love working with it, it’s perfect for us.
It’s our high throughput system.

Dr. Jorine Eeftens

Brangwynne Lab, Princeton University

Wavelength Availability

  • White
  • UV 365 nm
  • Violet 405 nm, 420 nm
  • Blue 450 nm, 470 nm
  • Green 525 nm
  • Yellow 590 nm
  • Red 630 nm, 660 nm, 740 nm
  • Infra-Red 940 nm

Two-color arrays are also available for alternating stimulation and inhibition.

LAD Array Drivers

LAD1 and LAD4 drivers are used to power our arrays. Pulse intensity and timing can be directly controlled by an analog input. The LAD can also be set to deliver constant illumination.

The LAD4 and LEDA4 allows you to split a 96 well plate into 4 sectors and illuminate each sector independently.

Pulse Generator for Optogenetics

STOmk-2 is a pulse generator developed for optogenetics. By connecting STOmk-2 to TRG port on the Teleopto Remote Controller via a trigger cable, you can control the timing of light stimulation by TTL pulses. Pulses are defined by the parameters illustrated below.

Currently being used to study

  • Stem cell (IPSC) differentiation
  • Developmental biology 
  • Molecular biology – receptor directed protein expression (via Cry2 activation)
  • CRISPR/Cas9 gene modification
  • Oncology 
  • Opthalmology & ophthalmologic drug development
  • Photostimulation and bleaching of proteins

Please see our optogenetic resource guide to help plan your experiments.

Publications


Duran Corbera, A., Catena, J., Otero Viñas, M., Llebaria, A., & Rovira, X. (2020). Photoswitchable antagonists for a precise spatiotemporal control of β2-adrenoceptors. Journal of Medicinal Chemistry.

Zhu, L., Richardson, T. M., Wacheul, L., Wei, M. T., Feric, M., Whitney, G., … & Brangwynne, C. P. (2019).  Controlling the material properties and rRNA processing function of the nucleolus using light. Proceedings of the National Academy of Sciences116(35), 17330-17335.

Westergard, T., McAvoy, K., Russell, K., Wen, X., Pang, Y., Morris, B., … & Haeusler, A. (2019).  Repeat‐associated non‐AUG translation in C9orf72‐ALS/FTD is driven by neuronal excitation and stress. EMBO molecular medicine11(2), e9423.

Frequently Asked Questions

Where can I find out more about how to plan my optogenetics experiment?

Please see our Optogenetics Resource Guide, where we have shared some of the many resources our customers have found useful for planning their optogenetics projects.

How long will it take for me to receive my order?
Normally the lead time will be 1 month.
What colors are available for the array?

White,  UV 365 nm, V (violet 405 nm), 405 nm, 420 nm, B (Blue 470 nm), G (Green 525 nm), Y (Yellow 590 nm), R (Red 630 nm), 660 nm, 740 nm, 850 nm, I (Infrared 940 nm), please ask for others.

What is the output for the array?

Output is continuously adjustable, the maximum irradiance varies for each color. The light power starts from zero and has step-less regulation.

You can control the power using the knob on the front panel of the LAD driver, or vary the voltage of an analog trigger pulse. Please see the manual for detailed information or ask Amuza. 

What are the dimensions of the LEDA-X array?
144 x 100 x 16 mm.
Do you know if the light power is uniform between and within each well in the 96 well plate? Most LEDs have a cone-like spread, so I was wondering if the cone covered the full surface of cells in each well.

Our LEDs have a quite wide-spreading angle (120 degrees), and so cover the full surface of each well in a 96 plate.

Are the arrays calibrated so all LEDs have the same power?
The typical light power variability between LEDs is about 10%.

What is the maximum voltage output of the LED Array Driver?
The maximum voltage of LAD-1 is 13.5V.
What is the length of the cable that connects the array to the driver?
2 meters, please let us know if you need longer cables.
Can we split the signal from one channel of the STO so that it controls 2 channels on the LAD4?
Yes. Just let us know and we will include the appropriate cable.
The VTS-4 can also be used to control the LAD4, correct?
VTS4 can only output 5V TTL, so you can control the stimulation timing, but you cannot modulate the intensity. Power can only be controlled by the knob on LAD-1 in this case.

Let's Get Started!