Eicom HPLC-ECD Applications

Developed and Tested at Eicom. We are able to provide technical support for all of our analytical applications because they were developed and tested extensively in Eicom’s own lab.

Step-by-step protocols. The application notes that are available on this page highlight each method. More detailed step-by-step protocols are provided to our customers.

Easy to switch between methods. If you need to use multiple methods on the same system, Eicom systems can easily be switched over because they have such a small, internal dead volume (thanks to the elimination of a pulse damper), and detector cells that stabilize quickly.

Application manuals and other documents can be downloaded for free on our support site. Login may be required.  

Microdialysis Sample Analysis

Highly Sensitive Acetylcholine Analysis – Cholinesterase Inhibitor Free
  • No acetylcholinesterase inhibitor required for the medial prefrontal cortex.
  • The new improved enzymatic column provides increased sensitivity
  • The reproducible sensitivity level of 5 fmol > chromatogram
  • 18 minute run time with an internal standard, 13 minutes without internal standard.
  • Also allows for the detection of Choline

See Application

Ultra Sensitive Analysis of Dopamine and Serotonin in 5 Min, Microdialysis Samples

*Find the best catecholamine analysis for your samples here.

See Application

The Simultaneous Analysis of Norepinephrine, Dopamine and Serotonin
  • Simultaneous analysis in 15 minutes
  • 260 Amol (50 fg) detection limit for DA and 580 Amol (0.1 pg) for NE and 5-HT
  • Allows for higher sample temporal resolution (15 min) > chromatogram

*Find the best catecholamine analysis for your samples here.

See Application

Rapid GABA Analysis by o-phthaldialdehyde Derivatization
  • Sensitivity level 30 fmol (1 nM x 30 µl).
  • High specificity. Ca2+ free perfusate decreases the peak height.
  • A 28-minute method with the incorporation of a switching valve > chromatogram

See Application

Glutamate and GABA Analysis in 12 Minutes
  • A flexible analytical method with adaptability to microdialysate and tissue homogenate
  • No UHPLC or two systems required.

See Application

Concise Glutamate Analysis by Using an Online Enzymatic Reaction
  • No precolumn derivatization required
  • A flexible analytical method with adaptability to microdialysate, blood, and tissue homogenate
  • Microdialysis sample run time of 10 minutes
  • The robust detection limit (< 3 fmol). (Microdialysis samples have higher concentrations) > chromatogram

See Application

Other Sample Analysis (Urine, Serum/Plasma, Tissue Homogenate)

Catecholamine Analysis in Plasma or Urine
  • Approx. 0.5 fmol (=0.1 pg) sensitivity. 20 min for one sample > chromatogram
  • Require sample preparation with activated alumina

* Find the best catecholamine analysis for your samples here

See Application

Analysis of Quinones such as CoQ10 and Vitamin K
  • No platinum black is required
  • Higher reproducibility
  • Lower running cost
  • We also have an application using platinum blacks.

See Application

Glutamate and GABA Analysis in 12 Minutes
  • A flexible analytical method with adaptability to microdialysate and tissue homogenate
  • No UHPLC or two systems required.

See Application

Concise Glutamate Analysis by Using an Online Enzymatic Reaction
  • No precolumn derivatization required
  • A flexible analytical method with adaptability to microdialysate, blood, and tissue homogenate
  • Microdialysis sample run time of 10 minutes
  • A robust detection limit (< 3 fmol). (Microdialysis samples have higher concentrations) > chromatogram

See Application

High Sensitive Thiols Detection using a Gold Working Electrode
  • New – ECD with a gold working electrode
  • Highly sensitive with 1 fmol detection limit for GSH and 0.5 fmol for -SH compounds > chromatogram

See Application

Detection of 3-Nitrotyrosine in Atmospheric Environments
  • For particulate matter (PM) and total suspended particles (TSP) sample analysis
  • Developed at Okayama University, Japan

See Application

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