Catecholamine Analysis Methods

Please use the information below to decide which application will best fit your individual needs. Or you can always contact us for help.

1. Determine your sample type:

  • Blood, Plasma, or Urine – Use the application for plasma shown here.
  • Tissue Homogenate – Use the application for tissue homogenates shown here.
  • Microdialysis – Follow the steps below.
  • Others – More information is required, contact us.

2. Determine which compounds you are interested in analyzing from microdialysis samples; norepinephrine (NE, noradrenaline),  dopamine (DA), serotonin (5-HT), MHPG, L-DOPA, DOPAC, NM, HVA and/or 5-HIAA.
Then determine which compounds you can compromise. Is the detection of both neurotransmitters and metabolites necessary? Additional detection of metabolites can result in decreased accuracy of neurotransmitters at lower concentrations or increase the analysis time. If detection of all or most of the previously listed compounds is required contact us. This application is available but not yet published on this website.

3. If you are interested in the detection of MHPG and NE, please contact us. This application is available but not yet published on this website.

4. Find the best application for your microdialysis samples from the table below. The analytical applications and corresponding neurotransmitter detection limits are listed below.

Application

NE (pg)

DA (pg)

5-HT (pg)

Run TimeRemarks
PP-ODS

N/A

0.03

0.03

5 min
CAX

0.1

0.05

0.1

15 minAntioxidants can interfere with the signal. Microdialysis-HPLC online analysis recommended
CA-5ODS

0.1

N/R

N/R

20 minDA, 5-HT will appear on the chromatogram but have a broad peak shape.
CA-5ODS

N/A

0.1

0.1

20 minDifferent mobile phase from the NE with CA-5ODS.

N/A: the peak is not available
N/R: the peak may appear but not recommended
For more detail about the CA-5ODS, please contact us

Questions?

Software that’s supported by us - to let you focus on your discoveries

You want to collect, analyze, and disseminate data—not provide software support! With our proven Task Studio software, simply assemble your trial with flowchart-like simplicity, and configure your triggers and executors with checklist-like ease. And if other researchers want to reproduce your findings, simply direct them to use Task Studio, too. This will save you from the headaches of “free software support” that you’d never intended to offer.