When it comes to neurotransmitter detection such as Dopamine, Serotonin, Norepinephrine, Glutamate, GABA, Acetylcholine, etc., in microdialysis samples, tissue homogenates, blood plasma, and others, there are three (3) main methods; LC-Mass Spectrometry (LC-MS), HPLC-ECD (Electrochemical Detection) & ELISA/Radioimmunoassays.
The first option is LCMS, it provides the highest sensitivity and specificity to the detecting neurotransmitters and also the most expensive option. The price range is between US$200,000 to $600,000.
The second method is HPLC-ECD which is much more cost affordable while offering great sensitivity. The price range is between US$20,000 to $60,000.
The third method is ELISA or Radioimmunoassays which offers less sensitivity. There is also a concern of selectivity due to cross-talk among antibodies.
Therefore, when choosing which method would work best for you, we would recommend HPLC-ECD.