Amuza’s Teleopto LEDA array: an incubator compatible illumination system for optogenetics, photobiology, and photochemistry in microplates.

Several years ago we found that while more and more work in optogenetics was being done in vitro, there was little in the way of equipment specifically designed to fill this role. Many labs built their own multiwell stimulation systems. These custom designs often performed brilliantly, but they took time to design and build and often made it more difficult to share protocols with other labs. We decided to provide a light source that is reproducible from well to well and from experiment to experiment, one that is ready to use in any lab.

The LEDA arrays are the result and now cell biologists, developmental biologists, chemists, neuroscientists, and others are using this array for optogenetics and to invent new techniques.

Original LEDA-B

Our most popular LED array is the LEDA-B: it is perfectly sized for a single 96 well plate. It has 96 LEDs with emission centered at a 470 nm wavelength. A lip surrounding the array locks your plate into place so that the wells stay centered over the LEDs. It can also be used with other microplates sharing the 96 well plate footprint. The cable is 2 m long, so only the array needs to be inside the incubator.

The LAD LED driver can be controlled manually, and you can vary the irradiance from zero up to roughly 1.5 mW/mm2, depending on wavelength. You can also use low voltage TTL pulses to trigger illumination. This way you can use a programmable pulse generator to control the LED array. Lowering the voltage of the pulse lowers the voltage sent to the LED array proportionately, allowing programmatic control of not just the timing but also the intensity of illumination. Our STO pulse generator is easy to program and use, but if you already have your own pulse generator or stimulator you should be able to use it with Teleopto. We now also offer programmable multichannel  LED drivers. These drivers do not require a pulse generator, they can be programmed via software on your PC.

Our LED arrays are available in many colors to match the ever-increasing variety of light-controlled tools available in life science, including:

White 

UV 280 and 365 nm

Violet 405 and 420 nm

Blue 450 and 470 nm

Green 525 nm

Yellow / orange 590 nm

Red 630, 660, & 740 nm

Infra-Red 850 & 940 nm

If you don’t see the wavelength you need listed on the Amuza website, please let us know, we may be able to find the correct LED for your application.

Our two-color arrays have two different color LEDs under each well. For example, a blue and yellow array allows you to work with step function (bistable) options: a blue pulse of light opens the channel and a yellow pulse closes it again.

Our LEDA4 and LEDA6 arrays allow you to control four or six different sections of the plate independently. This way you can trial different intensities or pulse trains simultaneously, saving your lab time and space when developing new protocols.

We also make these arrays in different sizes so that you can illuminate multiple plates at once, or change the placement of the LEDs to match the format of different well plates. These arrays are also perfect for illuminating Petri dishes, tissue culture bottles, flasks, and other vessels.

Our LED arrays are being used for a wide range of applications, including Cry2 based biomolecular condensates, developmental biology, Stem cell differentiation, Cardiac optogenetics, receptor activation, and protein expression, oncology, ophthalmology, development of novel opsins and other optogenetic tools, photochemistry, and neuroscience.

Questions?