Streamline 3D Bioprintings
–Let’s create tissues naturally.
Conventional technology uses scaffold-like polymer and exogenous materials to create 3D structures with embedded cells, known as bioinks, that mimic the Extracellular Matrix (ECM). Bioinks are not composed of 100% naturally produced ECM, and the cells in our bodies are not familiar with these exogenous materials. The novel Net Mold technology developed by TissueByNet takes into consideration cells’ biology by creating a natural support for 3D Bioprinting.
3D bioprinting performed naturally.
Naturally occurring ECM is secreted by cells to provide structural and biochemical support to our body tissues. In vitro tissue engineering technology uses the same biological principles by exploiting the ECM’s properties to build tissues. However, the largest challenge with this process is maintaining cells in a 3D structure before and during maturation of the ECM itself.
TissueByNet solved this problem.
To support cells at the early stages of 3D matrix construction during tissue development we created the Net Mold technology. The mold can be easily prepared by stacking of layers of thin metal combs to form a sieve or net. Subsequently, cell aggregates are dispensed into the molded pits without any artificial materials, Bioinks, or hydrogels.
Tissue Creation Outline – TissueByNet
Step 1. Creation of cell aggregates (spheroids).
Culture your cells in a hanging drop system. Cells will aggregate and form spheroid after about 5 days. This procedure creates many uniform spheroids.
Step 2. Fill in to pit created in Net Mold.
In the package of Net Mold, there are two types of stainless steel parts. One is fine comb and the others are coarse comb. Coarse works to create a space. Fine one will prevent spheroids from leaking out but allow culture media to access the spheroids.
Step 3. Maturation in shaker.
Giving shear stress to let cells secrete ECM. Normal incubation time is 5 to 10 days.
Step 4. Removing Net Molds.
Net Molds have special coating to prevent ECM from fusing. That is why these parts are for single use.
2018/8/8 Published Paper at JOVE.
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation. Bai, Y., Yeung, E., Lui, C., Ong, C. S., Pitaktong, I., Huang, C., Inoue, T., Matsushita, H., Ma, C., Hibino, N. A , e58252, doi:10.3791/58252 (2018).